alamarBlue^® 阿尔玛蓝（细胞增殖及毒性检测试剂）

基本描述：

阿尔玛蓝（Alamar Blue）是一种细胞增殖检测试剂，对各种人和哺乳动物细胞、细菌和真菌提供一种快速和敏感的细胞增殖和毒性检测方法。

阿尔玛蓝（Alamar Blue）是一种基于刃天青（resazurin）的检测试剂。刃天青作为一种一种氧化还原（REDOX）指示剂，根据细胞代谢还原情况发生颜色变化。氧化态的刃天青呈紫蓝色且基本无荧光，其还原态产物试卤灵（resorufin）转变为粉红色且高度荧光，产生的荧光强度与呼吸活细胞数量呈正比。通过检测呼吸过程中氧化水平，阿尔玛蓝用作一种定量检测细胞活力和毒性的直接指示剂。阿尔玛蓝的颜色变化可通过普通分光光度计检测吸光度变化，检测波长为570nm，参考波长为600nm；阿尔玛蓝的荧光变化可通过荧光光度计检测，激发波长在530~560nm之间，发射波长为590nm。

检测原理：（细胞增殖实验 Cell proliferation assay））

※ 生长中细胞引起Alamar Blue的化学还原反应；

※ 持续性细胞生长维持还原环境，使得Alamar Blue呈红色，发强荧光；

※ 生长受抑制维持氧化环境，使得Alamar Blue呈蓝色，无荧光；

※ 使用基于荧光或吸光值检测的仪器收集数据；

※ 荧光检测的激发波长为530~560nm之间，发射波长为590nm；

※ 吸光值检测的波长为570nm，参考波长为600nm；

保存方法：

室温12个月稳定，2-8°C保存20个月稳定；避光保存；

产品优势：

• 简单：水溶性，只需添加和测量即可；
• 灵活：显色和荧光检测，悬浮细胞和贴壁细胞；
• 无毒：对细胞无毒，对使用者和环境也无毒；
• 可靠：大量引用用于细胞毒性和活力检测；
• 规模化：简单放大体系用于高通量分析
• 高灵敏：zui低可检测到50个细胞
• 稳定高：*缓冲配方使其能用于长期研究；
• 经济：不需要细胞裂解，使细胞能继续培养用于后续分析；

订购信息：品牌保证，现货供应，大量使用文献，国内外上万实验室的认可品质，咨询，，：。

【注1】：按照96孔板，每孔100µl终体积来算，5ml alamarBlue^®可做500个孔；25ml可做2500个孔。

【注2】：alamarBlue^® Technical Datasheet

引用文献（AbdSerotech alamarBlue^®，43篇）：

1. Lewis, C.S. et al. (2010) Local Antibiotic Delivery with Bovine Cancellous Chips.
2. Alsford, S. and Horn, D. (2011) Elongator Protein 3b Negatively Regulates Ribosomal DNA Transcription in African Trypanosomes.
3.  Crilly, A. et al. (2011) Phosphodiesterase 4 (PDE4) regulation of proinflammatory cytokine and chemokine release from rheumatoid synovial membrane.
4. Paget, C. et al. (2011) Potential Role of Invariant NKT Cells in the Control of Pulmonary Inflammation and CD8+ T Cell Response during Acute Influenza A Virus H3N2 Pneumonia.
5. Lakhkar, N. et al. (2011) Titanium and strontium-doped phosphate glasses as vehicles for strontium ion delivery to cells.
6. Wilson, B.A. et al. (2011) High-throughput screen identifies novel inhibitors of cancer biomarker a-methylacyl coenzyme A racemase (AMACR/P504S).
7. Lau, L.I. et al. (2011) The Effect of Photooxidative Stress and Inflammatory Cytokine on Complement Factor H Expression in Retinal Pigment Epithelial Cells.
8. Arlian, B.M. and Tinker, J.K. (2011) Mucosal Immunization with a Staphylococcus aureus IsdA-Cholera Toxin A2/B Chimera Induces Antigen-Specific Th2-Type Responses in Mice.
9. Voloshin, T. et al. (2011) G-CSF supplementation with chemotherapy can promote revascularization and subsequent tumor regrowth: prevention by a CXCR4 antagonist.
10. Rao, T.D. et al. (2011) Dual-Fluorescence Isogenic High-Content Screening for MUC16/CA125 Selective Agents.
11. Uitdehaag, J.C. et al. (2011) Multidimensional Profiling of CSF1R Screening Hits and Inhibitors: Assessing Cellular Activity, Target Residence Time, and Selectivity in a Higher Throughput Way.
12. Rzhepishevska, O. et al (2011) The antibacterial activity of ga3+ is influenced by ligand complexation as well as the bacterial carbon source.
13. Xu, S. et al. (2011) Marek's disease virus type 1 microRNA miR-M3 suppresses cisplatin-induced apoptosis by targeting Smad2 of the transforming growth factor beta signal pathway.
14.  Ardakani, A.G. et al. (2014) Quantifying the correlation between spatially defined oxygen gradients and cell fate in an engineered three-dimensional culture model.
15. Nakayama, G.R. et al. (1997) Assessment of the Alamar Blue assay for cellular growth and viability in vitro.
16. Diril, M.K. et al. (2012) Cyclin-dependent kinase 1 (Cdk1) is essential for cell division and suppression of DNA re-replication but not for liver regeneration.
17.  Warrier, T. et al. (2012) Antigen 85C inhibition restricts Mycobacterium tuberculosis growth through disruption of cord factor biosynthesis.
18. Dreidax, D. et al. (2013) Low p14ARF expression in neuroblastoma cells is associated with repressed histone mark status, and enforced expression induces growth arrest and apoptosis.
19. Wang, H. et al. (2014) Enhanced osteoblast responses to poly ether ether ketone surface modified by water plasma immersion ion implantation.
20. Park, K.H. et al. (2014) Expression of polysialylated neural cell adhesion molecules on adult stem cells after neuronal differentiation of inner ear spiral ganglion neurons.

相关产品：

 — —Written/Edited by V. Shallan【版权归MKBio懋康所有】
 

上海懋康生物科技有限公司是一家涉足于生命科学和生物技术领域研究的试剂、仪器和实验室消耗品与实验服务工作，主要从事细胞生物学、植物学、分子生物学、免疫学、生物化学、蛋白组学。生物制药与诊断试剂研发生产等领域。 本公司秉承“以人为本，以诚为信、合同守信”的经营理念。坚持"品质保障"的原则为广大客户提供优质产品。