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懋康生物重点推出染色体荧光探针-色霉素A3(CA3)

Chromomycin A3 色霉素A3

 

搜索关键词:

Chromomycin A3 色霉素A3Hoechst 33342 细胞核探针; Chromosome-banding 染色体分带;Protamine deficiency 鱼精蛋白缺乏;荧光DNA染料;CAS7059-24-7

订购信息:【高品质进口原料,现货供应】:

产品名称

产品编号

规格

CAS NO.

价格(元)

Chromomycin A3 色霉素A3

MX4218-1MG

1mg

7059-24-7

880

Chromomycin A3 色霉素A3

MX4218-5MG

5mg

7059-24-7

2980

产品描述:

色霉素A3Chromomycin A3CMA3CAS7059-24-7,同义名Aburamycin b,由灰色链霉菌Streptomyces griseus)代谢纯化所得的一种蒽醌抗生素,具有抗菌、抗真菌和抗肿瘤活性。常用作一种荧光DNA染料,非常适合用来筛查经子染色质存在的精蛋白缺乏(protamine deficiency)。在二价金属离子存在的条件下,色霉素A3CMA3)通过与DNA特异性结合与可逆作用来阻断大分子合成。另外,通过结合到DNA小沟,色霉素A3有效调控与DNA促旋酶的竞争性抑制,从而显著影响拓扑异构酶II活性。CMA3对非小细胞性肺癌和宫近癌呈现体外细胞毒性,作用于HCC44A549ME180 HeLa细胞的IC50s分别是1426040nMCMA3还能通过改善Sp1Sp3转录因子结合来抑制氧化应激和DNA损伤诱导的神经损伤。具体内活性。

产品特性

1)   CAS NO7059-24-7

2)   化学名:3B-O-(4-O-acetyl-2,6-didexy-3-C-methyl-α-L-arabino-hexopyranosyl)-7-methylolivomycin D

3)   同义名:Toyomycin, Aburamycin B, Antibiotic 69895 A, Antibiotic B 599, NSC-58514

4)   分子式:C57H82O26

5)   分子量:1183.25

6)   纯度:>97%HPLC

7)   外观:类白色至黄色粉末

8)   Ex/Em Max445/575 nm

9)   溶解性:溶于乙醇(~20mg/ml),DMSO~20mg/ml),乙酸乙酯(10mg/ml

10)化学结构式:

产品使用【来自文献,仅做参考】

文献1Esterhuizen AD et al. Chromatin packaging as an indicator of human sperm dysfunction. J Assist Reprod Genet. 2000 Oct;17(9):508-14. PMID: 11155324

体外研究:

样本类型(Sample type):Semen Preparation

应用目的(Purpose):Using CMA3 staining to determine quality chromatin packaging

实验方法(Assay):CMA3 staining thin smears were prepared, air dried, and fixed in methanol/acetic acid 3:1 at room temperature for 20 min. The slides were air-dried and stained with 60–100µl CMA3 in a dark chamber for 20 min. Slides were then washed in McIlvaine buffer and mounted using Dabco. Two hundred spermatozoa were evaluated under a Nikon fluorescent microscope.

文献2Iranpour FG et al. Chromomycin A3 staining as a useful tool for evaluation of male fertility. J Assist Reprod Genet. 2000 Jan;17(1):60-6. PMID: 10754785

体外研究:

样本类型(Sample type):Semen aliquots

应用目的(Purpose):Using CMA3 staining to assess the packaging quality of the chromatin in sperm

实验方法(Assay):The spermatozoa were washed again, fixed in methanol/glacial acetic acid (3:1) at 4℃ for 5 min and then spread on slides. For CMA3 staining, each slide was treated for 20 min with 100µl of CMA3 solution (0.25 mg/ml in McIlvaine buffer, pH 7.0, containing 10 mM MgCl2). The slides were then rinsed in buffer and mounted with buffered glycerol. Microscopic analysis of the slides was performed on a fluorescent Zeiss axioplane microscope with the appropriate filters.

文献3Toepfer N et al. Atorvastatin induces autophagy in prostate cancer PC3 cells through activation of LC3 transcription. Cancer Biol Ther. 2011 Oct 15;12(8):691-9. PMID: 21768780

体外研究:

细胞类型(Cell type):PC3 cells

药物配制(Preparation):Chromomycin A3 was dissolved in ethanol as 1:1000 stock solution.

实验方法(Assay):PC3 cells were seeded at indicated densities in 12-well plates in triplicates and treated with DMSO, atorvastatin (10 μM), cyclohexamide (10 μg/ml) and chromomycin A3 (5 μM) or atorvastatin plus cyclohexamide or chromomycin A3. The cells were cultured for 2, 3 and 4 d, trypsinized, and counted by the cell counter Cellometer Auto T4.

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注意事项

1)   本品具有一定的致癌和致畸活性,操作过程中请注意防护,避免直接接触。

2)   为了您的安全和健康,请穿实验服并戴一次性手套操作。

 

 — —Written/Edited by V. Shallan【版权归MKBio懋康所有】

 

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