Chromomycin A3 色霉素A3
搜索关键词:
Chromomycin A3 色霉素A3;Hoechst 33342 细胞核探针; Chromosome-banding 染色体分带;Protamine deficiency 鱼精蛋白缺乏;荧光DNA染料;CAS:7059-24-7
订购信息:【高品质进口原料,现货供应】:
产品名称 | 产品编号 | 规格 | CAS NO. | 价格(元) |
Chromomycin A3 色霉素A3 | MX4218-1MG | 1mg | 7059-24-7 | 880 |
Chromomycin A3 色霉素A3 | MX4218-5MG | 5mg | 7059-24-7 | 2980 |
产品描述:
色霉素A3(Chromomycin A3,CMA3,CAS:7059-24-7),同义名Aburamycin b,由灰色链霉菌(Streptomyces griseus)代谢纯化所得的一种蒽醌抗生素,具有抗菌、抗真菌和抗肿瘤活性。常用作一种荧光DNA染料,非常适合用来筛查经子染色质存在的精蛋白缺乏(protamine deficiency)。在二价金属离子存在的条件下,色霉素A3(CMA3)通过与DNA特异性结合与可逆作用来阻断大分子合成。另外,通过结合到DNA小沟,色霉素A3有效调控与DNA促旋酶的竞争性抑制,从而显著影响拓扑异构酶II活性。CMA3对非小细胞性肺癌和宫近癌呈现体外细胞毒性,作用于HCC44,A549,ME180和 HeLa细胞的IC50s分别是1,42,60和40nM。CMA3还能通过改善Sp1和Sp3转录因子结合来抑制氧化应激和DNA损伤诱导的神经损伤。具体内活性。
产品特性
1) CAS NO:7059-24-7
2) 化学名:3B-O-(4-O-acetyl-2,6-didexy-3-C-methyl-α-L-arabino-hexopyranosyl)-7-methylolivomycin D
3) 同义名:Toyomycin, Aburamycin B, Antibiotic 69895 A, Antibiotic B 599, NSC-58514
4) 分子式:C57H82O26
5) 分子量:1183.25
6) 纯度:>97%(HPLC)
7) 外观:类白色至黄色粉末
8) Ex/Em Max:445/575 nm
9) 溶解性:溶于乙醇(~20mg/ml),DMSO(~20mg/ml),乙酸乙酯(10mg/ml)
10)化学结构式:
产品使用【来自文献,仅做参考】
文献1,Esterhuizen AD et al. Chromatin packaging as an indicator of human sperm dysfunction. J Assist Reprod Genet. 2000 Oct;17(9):508-14. PMID: 11155324 体外研究: 样本类型(Sample type):Semen Preparation 应用目的(Purpose):Using CMA3 staining to determine quality chromatin packaging 实验方法(Assay):CMA3 staining thin smears were prepared, air dried, and fixed in methanol/acetic acid 3:1 at room temperature for 20 min. The slides were air-dried and stained with 60–100µl CMA3 in a dark chamber for 20 min. Slides were then washed in McIlvaine buffer and mounted using Dabco. Two hundred spermatozoa were evaluated under a Nikon fluorescent microscope. |
文献2,Iranpour FG et al. Chromomycin A3 staining as a useful tool for evaluation of male fertility. J Assist Reprod Genet. 2000 Jan;17(1):60-6. PMID: 10754785 体外研究: 样本类型(Sample type):Semen aliquots 应用目的(Purpose):Using CMA3 staining to assess the packaging quality of the chromatin in sperm 实验方法(Assay):The spermatozoa were washed again, fixed in methanol/glacial acetic acid (3:1) at 4℃ for 5 min and then spread on slides. For CMA3 staining, each slide was treated for 20 min with 100µl of CMA3 solution (0.25 mg/ml in McIlvaine buffer, pH 7.0, containing 10 mM MgCl2). The slides were then rinsed in buffer and mounted with buffered glycerol. Microscopic analysis of the slides was performed on a fluorescent Zeiss axioplane microscope with the appropriate filters. |
文献3,Toepfer N et al. Atorvastatin induces autophagy in prostate cancer PC3 cells through activation of LC3 transcription. Cancer Biol Ther. 2011 Oct 15;12(8):691-9. PMID: 21768780 体外研究: 细胞类型(Cell type):PC3 cells 药物配制(Preparation):Chromomycin A3 was dissolved in ethanol as 1:1000 stock solution. 实验方法(Assay):PC3 cells were seeded at indicated densities in 12-well plates in triplicates and treated with DMSO, atorvastatin (10 μM), cyclohexamide (10 μg/ml) and chromomycin A3 (5 μM) or atorvastatin plus cyclohexamide or chromomycin A3. The cells were cultured for 2, 3 and 4 d, trypsinized, and counted by the cell counter Cellometer Auto T4. |
相关产品:
货号 | 产品名称 | Ex/Em(nm) DNA-bound | 规格 | 价格(元) |
MX4201-10MG | Hoechst 33258 细胞核探针 | 352/461 | 10mg | 179 |
MX4203-10MG | Hoechst 33342 细胞核探针 | 350/461 | 10mg | 179 |
MS0027-1MG | Mithramycin A 光辉霉素 | 450/570 | 1mg | 510 |
MS0027-5MG | Mithramycin A 光辉霉素 | 450/570 | 5mg | 1780 |
MS0035-100MG | Cycloheximide 放线均酮(环己亚胺) | / | 100mg | 350 |
注意事项
1) 本品具有一定的致癌和致畸活性,操作过程中请注意防护,避免直接接触。
2) 为了您的安全和健康,请穿实验服并戴一次性手套操作。
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